DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Adjusting the Sensitivity of the Plate Sensor Switch
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-39
Testing the
Adjustment
1. Place the reaction plate in the input stack 1 of the Plate Handler.
2. Power on the 7900HT instrument, the Plate Handler, and the computer.
3. Select > Programs > Zymark Twister Plate Handler > Twister.
The Zymark Twister Software starts.
4. Click Manual Control.
5. In the Manual Control dialog box, click stack 4.
The Plate Handler arm moves over the input stack.
6. Click .
If the adjustment was successful, the Plate Handler arm will lower upon the
plate until the plate detector switch engages confirming the presence of the
plate.
If Plate Handler arm emits a grinding sound, adjust the plate sensor switch:
a. In the Zymark Twister Software, click to raise the Plate
Handler arm.
b. Turn the thumbscrew in the Down direction 10 steps.
c. Repeat step 6 until the Plate Handler arm successfully detects the plate.
7. Click , then click .
If the adjustment was successful, the Plate Handler arm will grasp the plate and
remove it from the plate stack.
If Plate Handler arm stops before the gripper fingers are able to contact the plate
and fails to grasp or pick up the plate, adjust the plate sensor switch:
a. Turn the thumbscrew in the Up direction 10 steps.
b. Grasp the plate with one hand and, from the Zymark Twister Software,
click to release the plate.
c. Replace the reaction plate into input stack 1 of the Plate Handler.
8. Repeat steps 6 through 7 until the Plate Handler arm successfully retrieves the
plate.
9. Grasp the plate with one hand and, from the Zymark Twister Software,
click to release the plate.
Click