BD FACSAria - Page 269

Index 269
doors, sort collection chamber 35
doublets
discrimination experiment 105
discrimination gating 136
eliminating 146
drop
breakoff 63
charging 70
conflicts 71
correction factors 69, 157
deflection 71
delay 68, 69, 158
See also Accudrop.
satellites 111
Drop 1
about 64, 66
adjusting 66, 111
drop delay, automatic method 162
E
editing
sort layouts 100
statistics view 147
electrical safety xv
electronics
aborts 235
about 42
troubleshooting 238
emergency stop button 43
emission spectra 51
emptying waste xvi, 115
error messages
cleaning mode failure 237
cytometer disconnected 238
cytometer not responding 238
Master DAQ overflow 238
events
not showing in plots 230, 232
rate, troubleshooting 233, 234
troubleshooting 235
excitation optics 36
Experiment Layout 143
experiments
Accudrop 105, 158
creating 123
cytometer QC 105
doublet discrimination 105
QC, setting up 122
reusing 133
setting up 143
sorting 165
templates 105
exporting sort reports 104, 168
F
FACSAria See BD FACSAria.
FACSDiva software See software.
ferrules, removing 201
fiber optics 37
filling containers 114
filters
about 53
air, changing 203
bandpass 55
bubble, bypassing 186, 193
changing 199, 203, 253
default setup 40, 250, 251
discriminating 55
fluid, changing 199
holders 242
longpass 54
neutral density (ND) 57, 241, 254
optical, cleaning 213
purging 198, 214
removing 199, 203, 254
Fine Tune mode 76
flow cell
about 30