Run the dilution plate
IMPORTANT! Do not run the dilution plate as a calibration.
1.
L
oad the plate into the instrument.
CAUTION! The instrument should be used by tr
ained operators who have been warned of the
moving parts hazard.
2.
Set up a genotyping plat
e file on the instrument (see “Create and run a plate from a system
template or a saved plate file” on page 47).
To use a system template for genotyping, see “System template file names” on page 34. Select a
genotyping method that is compatible with the Master Mix.
3.
In the Method tab, set the hold to 60°C for 2 minutes.
4.
In the Plate tab, enter the dilution series information for the appropriate wells.
5.
Tap Start run.
6.
When the run is complete, transfer the data files for analysis (see “Transfer a data file immediately
after a run” on page 81 or “Transfer data files at a later time” on page 81).
7.
Unload the plate from the instrument.
CAUTION! PHY
SICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smar
t Help if the instrument does not eject the plate (see “Request technical support
with Smart Help” on page 172).
Determine the optimal dye concentration
Use the Connect platform or the deskt
op software to review the dye signal data and select the dilution
to use for dye calibration.
1.
In the Results tab, select Raw data plot.
This plot displays the raw fluorescence signal of each optical filter, for individual wells.
Note: The raw data plot cannot be viewed on the instrument touchscreen.
2.
F
or each replicate population of dilutions, select the wells in the plate layout to view in the plot.
Chapter 7 C
alibrate and verify instrument performance
Calibrate custom dyes
7
116
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
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