Add a custom dye to the instrument
1.
In the home screen, tap (Settings)4Maintenance and service4Calibration4Custom dye.
The Custom Dye screen is displayed.
2.
Tap Add custom dye.
3.
Tap the Custom Dye Name field, then enter a name.
IMPORTANT!
·
Do not use a syst
em dye name for a custom dye name.
·
Dye names are spacing sensitive and cannot contain special characters.
4.
Select the type of dye.
Option Description
Reporter The dye works in conjunction with a quencher dye to report an increase of PCR product.
Quencher The dye suppresses the fluor
escence of a reporter dye until amplification of PCR product.
Both The dye can be used as a reporter dye or a quencher dye.
5.
T
ap Save.
IMPORTANT! The cust
om dye must also be added to the desktop software library before creating,
running, or analyzing experiments that use custom dyes.
Perform a custom dye calibration
1.
In the instrument home scr
een, tap
(Settings)4Maintenance and
service4Calibration4Custom dye.
The Custom Dye screen is displayed.
2.
Tap a custom dye.
3.
Review the information, then tap Update.
4.
Enter the calibration temperature.
Note: The default calibration temperature is set to 60°C.
5.
(Optional) T
ap Reagents, then enter the reagent information.
6.
Load the plate into the instrument, then tap Start.
CAUTION! The instrument should be used by tr
ained operators who have been warned of the
moving parts hazard.
Calibration complete is displayed when the run is complet
e.
Chapter 7 Calibrate and verify instrument performance
Calibrate custom dyes
7
118
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
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