Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Page 121

5.
Load the plate into the instrument
6.
Tap Start.
7.
When the run is complete and the screen displays Calibration complete, tap View
results4Details.
8.
Review the plot.
Passing calibration results show uniform signals with peaks that are aligned with the dye
wavelength.
Figure 18 An example dye calibration plot
Not
e: The peaks for your dye may align with a dierent filter set.
9.
Select an action depending on whether the cust
om dye calibration passed or failed.
Calibration
status
Action
Passed
1.
T
ap Accept Results or Reject Results.
Note: Accepting the results saves the calibration data to the instrument and
overwrites existing data.
2.
(Optional) T
ap Transfer EDS to transfer the calibration data to a USB drive.
Failed
1.
Perform the calibration again using a new custom dye plate. Create the plate
using the next dye concentration greater than the optimal dye concentration (see
“Determine the optimal dye concentration” on page 116).
2.
For more information, see “Troubleshoot calibration failure” on page 95.
10.
Unload the plat
e from the instrument.
Note: You must also add the custom dye to the desktop software dye library before you create, run, or
analyze experiments that use custom dyes.
Note: T
o perform a custom melt experiment, you can either create a Standard Curve or a Custom
experiment with melt, then specify the data points per degree in the method.
Chapter 7 Calibrate and verify instrument performance
Calibrate for a custom melt curve run
7
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
121