Applied Biosystems 7900HT - Page 210

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Chapter 6 Analyzing Real-Time Data
6-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Table 6-1 Summary of Expression Levels
Detector
Sample
∆ C
T
s
Then expression
level is…
Example (for a 40-cycle experiment)
Calibrator Test Calib ∆C
T
Tes t ∆C
T
Result
Targ et
Endo
<
a
<
<
<
Displayed 25
33
32
35
• RQ is displayed
• Bar is displayed
Targ et
Endo
Max
b
<
<
<
A minimum
(bar displays ↑)
—
b
35
34
30
• RQ is displayed
•Bar displays↑
Targ et
Endo
<
<
Max
<
A maximum
(bar displays ↓ )
33
33
—
33
• RQ is displayed
•Bar displays↓
Targ et
Endo
Max
Max
Max
Max
Undetermined
(not displayed)
—
—
—
—
• RQ value is not
calculated
• Bar is not displayed
Targ et
Endo
<
Max
Max
Max
Undetermined
(not displayed)
30
—
—
—
Targ et
Endo
Max
Max
<
Max
Undetermined
(not displayed)
—
—
30
—
Targ et
Endo
Max
Max
Max
<
Undetermined
(not displayed)
—
—
—
30
Targ et
Endo
Max
<
Max
Max
Undetermined
(not displayed)
—
30
—
—
Targ et
Endo
<
Max
<
Max
Undetermined
(not displayed)
30
—
20
—
Targ et
Endo
<
Max
Max
<
Undetermined
(not displayed)
35
—
—
33
Targ et
Endo
<
<
Max
Max
Undetermined
(not displayed)
30
20
—
—
Targ et
Endo
Max
Max
<
<
Undetermined
(not displayed)
—
—
30
20
Targ et
Endo
Max
<
Max
<
Undetermined
(not displayed)
—
20
—
20
Targ et
Endo
Max
<
<
Max
Undetermined
(not displayed)
—
33
35
—
Targ et
Endo
<
<
<
Max
Undetermined
(not displayed)
30
35
33
—
Targ et
Endo
<
Max
<
<
Undetermined
(not displayed)
33
—
30
35
a< : Indicates that the sample C
T
is less than the maximum PCR cycle of the run (a valid C
T
).
bMax (or —): Indicates that the amplification curves for the associated sample replicate group never crossed the threshold
during the duration of the run (‘undetermined’ values).