Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Chapter 6 Analyzing Real-Time Data
6-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Table 6-1 Summary of Expression Levels
Detector
Sample
∆ C
T
s
Then expression
level is…
Example (for a 40-cycle experiment)
Calibrator Test Calib ∆C
T
Tes t ∆C
T
Result
Targ et
Endo
<
a
<
<
<
Displayed 25
33
32
35
• RQ is displayed
• Bar is displayed
Targ et
Endo
Max
b
<
<
<
A minimum
(bar displays ↑)
—
b
35
34
30
• RQ is displayed
•Bar displays↑
Targ et
Endo
<
<
Max
<
A maximum
(bar displays ↓ )
33
33
—
33
• RQ is displayed
•Bar displays↓
Targ et
Endo
Max
Max
Max
Max
Undetermined
(not displayed)
—
—
—
—
• RQ value is not
calculated
• Bar is not displayed
Targ et
Endo
<
Max
Max
Max
Undetermined
(not displayed)
30
—
—
—
Targ et
Endo
Max
Max
<
Max
Undetermined
(not displayed)
—
—
30
—
Targ et
Endo
Max
Max
Max
<
Undetermined
(not displayed)
—
—
—
30
Targ et
Endo
Max
<
Max
Max
Undetermined
(not displayed)
—
30
—
—
Targ et
Endo
<
Max
<
Max
Undetermined
(not displayed)
30
—
20
—
Targ et
Endo
<
Max
Max
<
Undetermined
(not displayed)
35
—
—
33
Targ et
Endo
<
<
Max
Max
Undetermined
(not displayed)
30
20
—
—
Targ et
Endo
Max
Max
<
<
Undetermined
(not displayed)
—
—
30
20
Targ et
Endo
Max
<
Max
<
Undetermined
(not displayed)
—
20
—
20
Targ et
Endo
Max
<
<
Max
Undetermined
(not displayed)
—
33
35
—
Targ et
Endo
<
<
<
Max
Undetermined
(not displayed)
30
35
33
—
Targ et
Endo
<
Max
<
<
Undetermined
(not displayed)
33
—
30
35
a< : Indicates that the sample C
T
is less than the maximum PCR cycle of the run (a valid C
T
).
bMax (or —): Indicates that the amplification curves for the associated sample replicate group never crossed the threshold
during the duration of the run (‘undetermined’ values).

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English