Applied Biosystems 7900HT - Page 96

DRAFT
September 1, 2004 11:39 am, CH_Run.fm
Chapter 3 Preparing a Run
3-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
4. Click .
The software saves the new detector and displays it in the detector list.
Note: If using the SDS Enterprise Database, you cannot create a detector that
that uses the same combination of detector name, reporter dye, and quencher
dye as an existing detector.
5. Repeat steps 2 through 4 to create detectors for all remaining assays on the plate.
Note: Click the button for information on the Detector Manager dialog box
or to view the procedures for editing, deleting, or searching for detectors.
6. Choose from the following:
Allelic Discrimination
Detector
Absolute or Relative
Quantification Detector
If constructing a
plate document for…
Then…
• absolute quantification
• relative quantification
• dissociation curve analysis
copy the detector(s) to the plate document as
explained in the procedure on page 3-13.
allelic discrimination create and apply markers to the plate
document as explained on page 3-14.