BD FACSAria - Page 272

272 BD FACSAria User’s Guide
changing 253
cleaning 213
default setup 40, 250, 251
optics
collection 38
cytometer 36
default setup 40
excitation 36
fiber 37
filter holders 242
reordering 240
stream-viewing 41
optimizing
cytometer performance 119
cytometer settings 135
drop delay 159
streams 156, 171, 172
options, cytometer 249
ordering supplies 239
O-ring, nozzle 206, 243
P
parameters
about 60
assigning 58, 87, 252
labels 143
measuring 60
scatter, distorted 234
pausing sorting 64, 101, 168
Performance 86
phase 69
Phase Masks
about 74
Yield Masks, using with 72, 75
photodiodes 58
photomultiplier tubes (PMTs)
about 39, 58
applying voltages 60
assigning 58, 87, 252
optimizing for application
settings 262
plate voltage 68
plates
deflection 33, 156
installing 170
sorting into 169
plenum
about 28
cleaning 190
plots
excessive debris 235
no events in 230
unexpected events in 232
PMTs
see photomultiplier tubes
populations
sorting 70, 98, 164, 166
troubleshooting 228, 235
power
buttons 42
cord xv
cytometer 42
fluidics cart 23, 25
laser 42
precaution labels xviii
precision modes 71, 75
See also sort precision modes.
preferences, default gate color 147
pressure
air 22, 25
sample 48
sheath 46, 82
troubleshooting 237
primary laser, verifying area scaling 124
priming fluids 115, 185
printing
sort reports 104, 168
worksheets 149
pulse, electronic 59