3 • Operation and Maintenance
Doc. 065051-03 1/08 99
System #1 Backpressure Calculations:
A 5 mL syringe and a syringe speed of 2 is assumed for this example.
Conclusion for system #1: The total backpressure of 273 kPa is less than
690 kPa. Therefore, the selected tubing IDs, tubing lengths, and syringe speed can
be used.
System #2 Backpressure Calculations:
A 5 mL syringe and a syringe speed of 2 is assumed for this example.
Conclusion for system #2: The total backpressure of 266 kPa is less than
690 kPa. Therefore, the selected tubing IDs, tubing lengths, and syringe speed can
be used.
Tubing Tubing
Length
(cm)
Backpressure
from tubing
(kPa/mL/min/cm)
Flow Rate
(mL/min)
Backpressure
Produced
(kPa)
Needle Seal Line
0.254 mm (0.010 in)
46 1.60 2.40 177 kPa
Diverter Line #1
0.330 mm (0.013 in)
38 0.55 2.40 50 kPa
5 μL Loop
0.254 mm (0.010 in)
12 1.60 2.40 46 kPa
Total backpressure for system #1 273 kPa
Tubing Tubing
Length
(cm)
Backpressure
from tubing
(kPa/mL/min/cm)
Flow Rate
(mL/min)
Backpressure
Produced
(kPa)
Needle Seal Line
0.254 mm (0.010 in)
46 1.60 2.40 177 kPa
Diverter Line #1
0.330 mm (0.013 in)
64 0.55 2.40 85 kPa
25 μL Loop
0.508 mm (0.020 in)
15 0.10 2.40 3.6 kPa
Total backpressure for system #2 266 kPa
To Next Page
Loading...
