Dionex As - Guidelines for Sample Volumes Used, Loop Sizes, and Injection Volumes

AS Autosampler Operator’s Manual
66 Doc. 065051-03 1/08
3.6.4 Guidelines for Sample Volumes Used, Loop Sizes, and
Injection Volumes
Sample Volumes Used for Each Type of Sample Loop Injection
Table 3-5 shows the volume of sample the AS aspirates from the sample
vial or well plate well and the volume of sample injected onto the column.
See Section 3.6.3
for descriptions of sample loop injection types and the
criteria used to determine which type of injection is performed.
The following tables give several examples for full-loop, large-loop, and
partial-loop injections. The examples show the amount of sample used
and injected for various loop sizes and injection volumes.
Injection Type Sample Volume Aspirated
from Vial or Well
Sample Volume
Injected
Full-loop
(2.5–16.9 μL loop)
Injection volume or
2.5 × loop size + 25 μL
(whichever is greater)
Loop size
Full-loop
(17.0–199.9 μL loop)
Injection volume or
4 × loop size
(whichever is greater)
Loop size
Large-loop
(200.0–7000.0 μL loop)
Injection volume or
1 × loop size + 500 μL
(whichever is greater)
Loop size
Partial-loop Injection volume + 2 × cut
volume
Injection volume
Partial-loop, limited-
sample
Injection volume Injection volume
Table 3-5. Sample Volumes Used for Each Injection Type