Dionex As - Removing Bubbles from the Syringe

AS Autosampler Operator’s Manual
218 Doc. 065051-03 1/08
B.3.14 Removing Bubbles from the Syringe
1. Fill a container with isopropyl alcohol.
2. Using a flathead screwdriver,
unscrew and remove the
syringe drive connecting screw
(see Figure B-51
).
3. Hold the syringe at the top and
bottom and unscrew it from
the valve.
4. Point the threaded end up into
the air. Push the syringe
plunger up and pull down
rapidly with short movements
to dislodge the bubbles. Repeat
until all bubbles are dislodged.
5. Place the threaded end of the
syringe into the isopropyl
alcohol and slowly pull out the
plunger to draw a full syringe
volume into the syringe.
Figure B-51. Removing the Syringe
6. Keeping the syringe in the liquid, slowly push the syringe plunger
until all bubbles exit the syringe. Then fill the syringe.
7. Alternatively, fill the syringe with isopropyl alcohol and then remove
the syringe from the liquid. Hold the syringe with the threaded end up
under a stream of deionized water. Push the syringe plunger up and
pull down until the bubble is removed.
8. Remove the syringe from the liquid. While holding it vertically, push
a small amount of liquid out the top to make sure there is no air
present.
9. Position the syringe under the valve.
10. Holding the syringe at the top and bottom, screw it back into the valve
and tighten fingertight.
Syringe Drive
Connecting
Screw (Remove)