Dionex As - Concentrate Injections

AS Autosampler Operator’s Manual
82 Doc. 065051-03 1/08
3.6.10 Concentrate Injections
The AS must be in concentrate mode to perform a concentrate injection
(see Section 3.6.2
). In concentrate mode, the AS draws the volume to be
injected from the sample vial and delivers it to a concentrator column (see
Figure 3-24
). The example in Figure 3-24 also shows the reagent prime
and reagent flush steps for matrix elimination.
Figure 3-24. Concentrate Injection Sequence (Example for Matrix Elimination)
1. Draw air
3. Draw air again to
isolate the sample
7. Inject sample
onto column
INJECT
Pump
Column
Waste
Waste
2. Draw sample
(inject volume)
5
line
. Prime sampling
needle assembly
with reagent
Reagent
LOAD
Pump
Column
Waste
Port
Concentrator
Waste
6. Flush concentrator
with reagent (matrix
elimination)
LOAD
Pump
Column
Concentrator
Waste
Concentrator
Sample
Syringe
KEY
Eluent
ReagentAir
Sample
DI Water
Reagent
L
P
C
L
S
W
L
P
C
L
S
W
L
P
C
L
S
W
LOAD
Pump
Column
Waste
Concentrator
L
P
C
L
S
W
S
Sample
Air
Flush