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Dionex As - Chromeleon Xpress; Running a Sequence of Injections in Chromeleon or

Dionex As
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AS Autosampler Operator’s Manual
54 Doc. 065051-03 1/08
3.2.2 Running a Sequence of Injections in Chromeleon or
Chromeleon Xpress
This section provides a brief description of the steps required to set up and
run the autosampler with Chromeleon or Chromeleon Xpress. For
detailed instructions, refer to the Chromeleon or Chromeleon Xpress
Help.
1. Create a program that specifies the functions the autosampler (and
other system instruments) should perform at a specific time.
Programs can be created automatically (with the help of a software
wizard) or manually (by editing an existing program).
In addition to commands for loading and injecting the sample, a
program for the AS can include commands for setting up the
autosampler (syringe speed, sample needle height, etc.) and for
preparing the sample before injection (pipetting, mixing, flushing,
etc.).
2. Create a sequence (a list of injections). For each injection, specify the
vial or well position (see Section 3.4
), the injection volume, the
program to run, and other parameters. Sequences can be created
automatically (with the help of a software wizard) or manually (by
editing an existing sequence).
3. Load the sequence into a batch.
4. Start the batch.

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