Dionex As - Normal, Full-Loop Injections

AS Autosampler Operator’s Manual
70 Doc. 065051-03 1/08
3.6.5 Normal, Full-Loop Injections
For a normal, full-loop injection, the AS draws a volume of sample from
the sample vial or well plate well. The volume of sample drawn depends
on the loop size and the specified injection volume (see Section 3.6.4
for
details). A portion of the sample is flushed through the loop, the loop is
filled, and an additional portion remains before the loop. The sample in
the loop is then injected onto the column (see Figure 3-18
).
Figure 3-18. Full-Loop Injection Sequence
Sample
Syringe
1. Draw air
3. Draw air again to
isolate the sample
4. Prepare to load
sample (excess
sample used)
KEY
Eluent
Sample
LOAD
Pump
Column
INJECT
Pump
Column
6. Inject sample
onto column. Empty
syringe (remaining
sample + air bubble
)
INJECT
Pump
Column
Waste Waste Waste
Flush
Air
2. Draw sample
5. Deliver sample
to the loop (excess
sample sent to
waste)
L
P
C
L
S
W
L
P
C
L
S
W
L
P
C
L
S
W
Sample
Air
Flush