Dionex As - Sequential Concentrate Injections

AS Autosampler Operator’s Manual
90 Doc. 065051-03 1/08
3.6.12 Sequential Concentrate Injections
In sequential concentrate mode, the AS delivers sample to two separate systems. A concentrator column replaces
the sample loop on each injection valve. Each system is operated independently and can have a different sample
and sample volume injected. Figure 3-29
shows a sequential concentrate injection to system #1 with reagent prime
and reagent flush steps for matrix elimination.
Figure 3-29. Sequential Concentrate Injection Sequence (Example for Matrix Elimination)
1. Draw air 3. Draw air
again to isolate
the sample
2. Draw sample
(inject volume)
Sample
Syringe
LOAD
Pump
Column
Waste
Concentrator
L
P
C
L
S
W
In
j
ect Valve #1 In
j
ect Valve #2
Pump
Column
L
P
C
L
S
W
Waste
1
S
2
PP
P
Diverter Valve
(Inject Valve #1
selected)
LOAD
5
line
. Prime sampling
needle assembly
with reagent
Reagent
LOAD
Pump
Column
Waste
Port
Concentrator
Waste
L
P
C
L
S
W
1
S
2
PP
P
To Inject
Valve #2
Waste
KEY
Eluent
Reagent
Air
Sample
DI Water
Unselected Valve
6. Flush concentrator
with reagent (matrix
elimination)
LOAD
Pump
Column
Concentrator
Waste
1
S
2
PP
P
L
P
C
L
S
W
To Inject
Valve #2
7. Inject sample
onto column
INJECT
Pump
Column
Waste
Concentrator
L
P
C
L
S
W
1
S
2
PP
P
To Inject
Valve #2
Concentrator
Sample
Air
Flush