3 • Operation and Maintenance
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5. Specify the injection volume and the cut volume required for the type
of injection to be performed. The cut volume specifies a volume of
sample to be “cut” (or omitted) from each side of the sample during
the injection process. To set up a full-loop injection, see Section 3.6.5
.
For a partial-loop injection, see Section 3.6.7
, and for a partial-loop
limited-sample injection, see Section 3.6.8
.
Example Program for Sequential Mode
The following example Chromeleon or Chromeleon Xpress program
shows the AS commands for sample loading and injecting when the
autosampler is in sequential mode.
Sampler.AcquireExclusiveAccess ;The timebase acquires
exclusive control of the AS, to
prevent another timebase from
attempting to control the AS
DiverterValve.Position_1 ;Divert flow to system #1
0.000 Sampler_InjectValve.LoadPosition ;Switch the inject valve on
system #1 to Load
Load ;Load sample onto the loop
Wait CycleTimeState ;Wait for the end of the
specified Cycle Time
Sampler_InjectValve.InjectPosition ;Switch the inject valve on
system #1 to Inject
Inject ;Sweep the sample onto the
column
Wait InjectState ;Wait for the injection to finish
5.00 BeginOverlap ;Begin preparing the next
sample
Sampler.ReleaseExclusiveAccess ;Release control; another
timebase can now acquire
exclusive control of the AS
30.00 End
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