Dionex As - Page 100

AS Autosampler Operator’s Manual
88 Doc. 065051-03 1/08
Setting Up a Sequential Mode Injection
The AS must be equipped with the sequential injection option
(P/N 063294) in order to perform sequential injections. The following
setup steps are required:
1. Follow the instructions in Section B.3.6
to install the sequential
injection diverter valve, connect the AS inject port tubing to the
diverter valve, and connect the diverter valve to the injection valves
on two systems.
2. Calibrate the inject port volume of each system. This is the volume
from the inject port through the diverter valve to the injection valve
(see Section 5.9
).
3. On the
SYSTEM PARAMETERS screen, select SEQUENTIAL in the
SAMPLE MODE field (see Figure 3-27).
4. On the
PLUMBING CONFIGURATION screen, enter the sample loop size
installed on each valve (see Figure 3-28
).
Figure 3-27. System Parameters Screen: Sequential Mode
Figure 3-28. Plumbing Configuration Screen: Sequential Mode
Help Prompt
SYSTEM PARAMETERS
WAIT:
ENABLED
METHOD SYRINGE SPEED:
CUT SEGMENT VOLUME:
INJECT PORT VOLUME:
uL
uL
10
S1 43
3
SAMPLE MODE:
SEQUENTIAL
FLUSH SYRINGE SPEED:
4
uL
S2 47
Help Prompt
PLUMBING CONFIGURATION
LOOP SIZE V1:
SAMPLE SYRINGE VOLUME:
PREP SYRINGE VOLUME:
SAMPLE SYRINGE CONFIG:
5
25
250
READY
uL
uL
mL
PREP SYRINGE CONFIG:
READY
uL
LOOP SIZE V2:
100