Dionex As - Normal, Partial-Loop, Limited-Sample Injections

AS Autosampler Operator’s Manual
76 Doc. 065051-03 1/08
3.6.8 Normal, Partial-Loop, Limited-Sample Injections
For a partial-loop, limited-sample injection, the AS draws only the
volume to be injected from the sample vial. The AS positions the sample
in the loop and injects it onto the column (see Figure 3-22
).
Figure 3-22. Partial-Loop, Limited-Sample Injection Sequence
Sample
Syringe
1. Draw air
2. Draw sample
(injection volume)
3. Draw air again to
isolate the sample.
5. Prepare to load
sample (flush fluid +
air bubble is used)
KEY
Eluent
Sample
6. Deliver sample
to
the loop
(injection volume)
7. Inject sample
onto column. Empty
sample syringe (air
bubble + an
y
excess fluid
)
Flush
Air
4. Draw flush
fluid for cleaning
inject port.
LOAD
Pump
Column
INJECT
Pump
Column
INJECT
Pump
Column
Waste Waste Waste
L
P
C
L
S
W
L
P
C
L
S
W
L
P
C
L
S
W
Sample
Air
Flush