3 • Operation and Maintenance
Doc. 065051-03 1/08 77
Setting Up a Partial-Loop, Limited-Sample Injection
1. On the SYSTEM PARAMETERS screen (see Section C.6.5), select
NORMAL in the SAMPLE MODE field.
2. In the Chromeleon or Chromeleon Xpress sequence (see Figure 3-19
)
or in the front panel schedule (see Section D.3
), enter an injection
volume that is less than the volume of the sample loop installed on the
injection valve.
3. In the Chromeleon or Chromeleon Xpress program (see Figure 3-21
),
or on the
SYSTEM PARAMETERS screen, enter a cut volume of zero.
Example Partial-Loop, Limited-Sample Injection Parameters:
Sample loop size: 100
μL
Injection volume: 50
μL
Cut volume: 0
μL
To perform the injection, the AS aspirates 50
μL from the sample vial
(the inject volume) and then enough flush fluid is drawn into the
syringe to position the sample. The 50
μL of sample is positioned just
before the loop and is then loaded onto the loop and injected.
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