D • Front Panel Control
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D.5.3 Defining Sample Prep Steps
Overview
Use the SAMPLE PREP screen (see Figure D-6) to specify a sequence of
operations to be performed before the method’s timed events. Six
standard sample prep operations are available:
DELAY, NEEDLE, PIPET,
MIX, FLUSH, and WAIT. In addition, if the sample preparation option is
installed,
DISPENSE and DILUTE operations are added. If a concentrator is
installed,
REAGENT FLUSH and REAGENT PRIME operations are added.
• Except for WAIT, each operation can be performed multiple times, or
not at all.
• The sample prep steps must include a single wait step. The wait
operation can be disabled, however, from the
SYSTEM PARAMETERS
screen (see Section C.6.5
).
Sample Prep
Operation
Description
DELAY
Specifies for how long the AS waits before proceeding to the
next step in the sample prep phase.
NEEDLE
The height of the needle above the bottom of the vial.
PIPET
Moves a specified volume of sample from one vial to another.
MIX
Mixes the vial contents by repeatedly drawing and expelling a
volume of sample.
FLUSH
Flushes the needle with a specified volume.
This operation is independent from the flush that takes place
before each injection in a schedule (see Section 2.7.6
).
DISPENSE
(Sample prep option only) Moves a specified volume of reagent
from a reservoir to a vial.
DILUTE
(Sample prep option only) Dilutes a volume of concentrate with
a specified volume of diluent.
REAGENT PRIME
(Concentrate mode only) Primes the lines with reagent.
REAGENT FLUSH
(Concentrate mode only) Flushes the concentrator with reagent.
WAIT
The AS waits for a continue command before proceeding with
the method. The command can be from the front panel (pressing
Hold/Run) or a TTL input.
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