Dionex As - Creating Sequences, Programs, and Quantification Methods

AS Autosampler Operator’s Manual
224 Doc. 065051-03 1/08
B.5.2 Creating Sequences, Programs, and Quantification
Methods
Follow these guidelines when creating sequences, programs (PGM file),
and quantification methods (QNT file) for simultaneous injection:
• Create one sequence that includes calibration standards for both
systems and the unknown samples.
• Create a PGM file for the unknowns that includes commands for
controlling both systems.
• Calibrate each system separately by creating separate calibration
PGM files.
• Create a single QNT file that includes the components from both
systems.
Creating a Method File for Systems Sharing an AS
For simultaneous injection analyses, create a single method file with all
components for each detector channel included in one component list.
Use the Duplicate Column command on the Peak Table, Amount Table,
and Peak Tracking pages of the method to create two extra Ret. Time
columns; associate each column with a different channel.
NOTE To add a column, right-click in the Ret. Time
column and select the Columns>Duplicate Column
command on the context menu.
For detailed instructions, refer to Chromeleon Help.
In the following example Peak Table, the Peak Name column includes
the components from two detector channels: one for cations and one for
anions. The Ret. Time ECD_1 column is associated with the cation
detector channel and displays only the cations. The Ret. Time ECD_2