Dionex As - Calibrating the Inject Port Volume

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5.9 Calibrating the Inject Port Volume
Calibrate the inject port volume at the following times:
• After replacing the needle seal assembly
• If reproducibility between injections is poor
Two methods are available for calibrating the inject port volume:
• Automatic calibration using Chromeleon or Chromeleon Xpress (Release 6.8
SP2 or later)
• Manual calibration using the front panel screens
This section describes the calibration in Chromeleon or Chromeleon Xpress.
If Chromeleon or Chromeleon Xpress (Release 6.8 SP2 or later) is unavailable,
refer to Section D.9
for instructions on how to use the front panel screens to
calibrate the inject port volume.
Note for Sequential Injections
For sequential injections, the volume to be calibrated includes not just the needle
seal line, but the path through the diverter valve and the diverter tubing to the
injection valve (see Figure 5-26
).
Figure 5-26. Sequential Injections:
Inject Port Volume Flow Paths
1
S
2
PP
P
Needle Seal Line
Diverter Valve
Inject Port
Diverter Line
W
L
P
C
L
S
Inject Valve #1
Inject Valve #2
W
L
P
C
L
S
Diverter Line
Needle