3 • Operation and Maintenance
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Full-Loop Sample Volume Examples
For full-loop injections, if the injection volume is more than 4 times the
loop size, the full injection volume is aspirated. The excess aspirated
sample is flushed through the loop, but the amount injected is equal to the
loop size.
Large-Loop Sample Volume Examples
For large-loop injections, if the injection volume exceeds the loop size +
500
μl, the full injection volume is aspirated. The excess sample aspirated
is flushed through the loop, but the amount actually injected is equal to
the loop size.
Installed Loop
Size (
μL)
Injection
Volume from
Chromeleon
Sequence (μL)
Sample
Aspirated from
Vial or Well
(
μL)
a
a. Excess aspirated sample is used to flush the loop before and after injection.
Sample Volume
Injected onto
Column (μL)
10 10 40 10
10 35 40 10
10 60 60 10
25 25 100 25
25 75 100 25
25 150 150 25
Table 3-6. Full-Loop Sample Volume Examples
Installed Loop
Size (
μL)
Injection
Volume from
Chromeleon
Sequence (μL)
Sample
Aspirated from
Vial or Well
(
μL)
a
a. Excess aspirated sample is used to flush the loop before and after injection.
Sample Volume
Injected onto
Column (μL)
500 500 1,000 500
500 700 1,000 500
500 1,100 1,100 500
Table 3-7. Large-Loop Sample Volume Examples
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