Dionex As - Page 80

AS Autosampler Operator’s Manual
68 Doc. 065051-03 1/08
Partial-Loop and Partial-Loop, Limited-Sample Volume Examples
For partial-loop injections, the amount of sample aspirated is equal to the
injection volume + 2
times the cut volume. The amount injected is equal
to the injection volume.
For partial-loop, limited-sample injections, the amount of sample
aspirated and injected is equal to the injection volume.
Maximum Sample Loop Sizes and Injection Volumes
The maximum loop size allowed depends on which sample syringe and
sampling needle assembly are installed. If the sample loop is too large, the
sample volume drawn exceeds the syringe/sampling needle assembly
volume, causing sample to enter the flush bottle.To prevent sample from
contaminating the flush bottle, the sample loop size must not exceed the
values shown in Table 3-9
.
Installed
Loop Size
(
μL)
Injection
Volume from
Chromeleon
Sequence (μL)
Cut
Volume
(
μL)
a
a. The cut volume specifies a volume of sample to be “cut” (or omitted) from
each side of the sample during the injection process. See Section 3.6.3
for
details.
Sample
Aspirated
from Vial or
Well (μL)
Sample
Volume
Injected onto
Column (μL)
25 10
5
20 10
25 20
10
40 20
25 10
0
10 10
25 20
0
20 20
Table 3-8. Partial-Loop and Partial-Loop, Limited-Sample
Sample Volume Examples