Dionex As - Page 227

B • Installation
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6. If the sample preparation option is installed, follow these steps to
prime the lines from each installed reagent reservoir (see
Figure B-49
):
a. Select the reagent reservoir, enter 2000
μL in the Prime Volume
field, and press Prime.
b. Repeat Step 6
for each additional reagent reservoir.
7. When priming is complete, there are often small air bubbles in the top
area of the syringe. Remove the bubbles before proceeding. See
Section B.3.14
for instructions.
Suggested Priming Volumes
Sample Syringe Size Suggested Priming Volume
≤1,000 μL 2,000 to 2,500 μL
5,000 μL
9,999 μL
a
a. The maximum value that can be entered.
10,000 μL
19,998 μL
b
b. Run two priming cycles of 9,999 μL each.
Table B-2. Suggested Priming Volumes
(Sample Preparation Option Not Installed)
Reservoir Prep Syringe Size Suggested Priming Volume
Flush 250 μL
2,000
μL
500 μL
2,000
μL
1,000 μL
4,000
μL
2.5 mL
5,000
μL
5 mL
9,999 μL
a
a. The maximum value that can be entered.
10 mL
19,998
μL
b
b. Run two priming cycles of 9,999 μL each.
A, B, C, and D
c
c. Repeat the priming procedure for each reagent reservoir.
All 2,000 μL
Table B-3. Suggested Priming Volumes
(Sample Preparation Option Installed)