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Software
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C
7.6 Compactness Histogram
Click on the COMPACTNESS HISTOGRAM button to display a diagram that compares viable cell shapes to
that of an ideal sphere. The ratio between the cell circumference and the cell area is based on an ideal sphere
(4p), which is equivalent to the value >1< on the abscissa of the Cell Compactness Histogram. The extent of
variation between the cell shapes and an ideal sphere is reflected in values being greater than 1. The shape of a
cell is known as the Compactness.
If a significant number of cells (ordinate) have a high value, this could indicate improper handling of a sample.
The Compactness Histogram can be used to check the state of the cell culture, for example, if the stirring speed
is too high or nutrient composition is incorrect.
Figure 23: Compactness Histogram
This diagram is based on the compactness of all viable cells in the measurement.
Graphical Display of the Analysis Results
Compactness Histogram
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