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Software
C
C
10.5.3. Creating a New Cell Type
A new Cell Type must be created and saved via the Cell Type dialog box before the Live Operator dialog box can
be opened for modification of that Cell Type.
Proceed as follows:
1
Open the Cell Type List dialog box. Highlight the existing Cell Type upon which the new Cell Type should be
based.
Do not select the Cell Type option Illumination Test, if it is available. This Cell Type is designed for
service purposes only and is not intended for analysis of cells. The parameters for this Cell Type should
not be changed.
2
Click on the NEW button. A message will appear asking if the new Cell Type should be based on the
highlighted Cell Type in the list. Click on YES. The Cell Type dialog box will open. The LIVE OPERATOR button
will be inactive at this time.
If you choose NO, the Cell Type dialog box will open with no entries in the Cell Type, Focus Offset,
or Sedimentation Duration fields.
3
Fill in the information for:
c
The name of the new Cell Type.
c
Focus Offset: the offset from the ideal focus point.
The Focus Offset should be set to -25 to ensure best results for cell count and determination of
viability. Selecting another value for this parameter may lead to poor results.
c
Sedimentation Duration: the amount of time the cells are allowed to settle before chamber is scanned.
The default value for the Sedimentation Duration is 90 or 60 seconds, depending on the Cell
Type. This can be shortened depending on the Cell
Type. Consult a local Roche Service representative
before choosing other
sedimentation times.
4
Make sure that the IR Operator is set to CXHiResImageOperator.
Do not select either the IR Operator DBDMOperator or the IR Operator
IlluminationTestImageOperator. These IR Operators are designed for service purposes only and are
not intended for analysis of cells. Do not change the parameters for these Operators.
5
Click on the SAVE button. The Cell Type dialog box will automatically close. The Cell Type List dialog box will
be active again and include the just-created Cell Type in the list.
6
Highlight the row of the new Cell Type and click on the EDIT button. The Cell Type dialog box will reopen and
the LIVE OPERATOR button will be active.
7
Click on the LIVE OPERATOR button and the Live Operator dialog box will open (see Figure 34). Ensure that
the Cell Type to be modified is selected from the drop-down menu.
8
In the Data Source area, select the workarea, followed by the Reactor ID, Sample ID, and Data Set of the
measurement for which the image analysis should be improved. The parameters for the loaded measurement
are preset.
9
Select an image of the measurement in the Image ID section. This image will be loaded and displayed in the
dialog box in the upper right-hand corner with the living cells marked with a green circle and the dead cells
marked with a red circle.
10
Make the desired modifications on a single parameter first. As soon as a parameter has been modified,
reprocessing of the data is automatic (see “Description of the Individual Operator Parameters”). The image
display then shows the reprocessed image (see “Working with the Live Operator”).
11
When an optimized adjustment of the Operator parameters has been found, click on SAVE to confirm the
modification for the new Cell Type.
12
Click on CANCEL to close the Live Operator dialog box without saving the changes.
■
Detailed step-by-step instructions for creating new Cell Types is provided in the Cedex HiRes Analyzer User
Training Guide in Section C, “Analyzing Unusual Cells”.
Administrator Functions
Adjustment of the Image Analysis using the Live Operator
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