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Additional Applications
C
C
C Additional Applications
1 Monitoring Growth of Cells Over Time
1.1 Performing Measurements for Growth Curve Analysis
1
Immediately after subculturing the cell culture of interest, remove a sample and perform a full analysis with
regard to cell concentration and viability using the Cedex HiRes Analyzer as described in Section B Main
Application: Counting Cells and Determining Viability.
2
Measure the cell culture with regard to cell concentration and viability at regular intervals for 7 to 10 days.
Intervals between measurements should typically range between 18 and 28 hours.
3
Approximately 2 or 3 days after the cell culture has stopped increasing in viable cell concentration, the
culture can be discarded and a growth curve analysis prepared as described in the following section.
n
Many cell cultures reach their growth limits within 7 to 10 days after being subcultured, particularly if there
is no exchange of media or addition of nutrients during that time. However, the time needed for a cell culture
to reach maximum growth and either decline in cell concentration or reach senescence (the plateau phase of
a growth curve) can vary depending on the cell type and conditions in which the culture is cultivated.
1.2 Result Analysis
After all measurements have been performed as described above, prepare the Cultivation Time Chart (CTC)
for the analysis of measurement results and cell culture growth pattern as described in the following chapters:
c Create a CTC View
c
Add One or More Series of Measurements to the CTC View
c Create a CTC Chart Associated with the View to Analyze Pattern of Growth Over Time
c Work with the Exponential Regression Function to Determine the Specific Growth Rate and Doubling Time
of the Cell Culture Based on the Log Phase of Growth
Monitoring Growth of Cells Over Time
Performing Measurements for Growth Curve Analysis
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