15
Main Application: Counting Cells and Determining Viability
B
B
B Main Application:
Counting Cells and Determining Viability
1 Check the Analyzer Before Starting Measurements
Accurate measurement results obtained with the Cedex HiRes Analyzer depend on correctly filled reagent
containers and the proper performance of the Analyzer. Before starting measurements, perform the following
checks:
1
Check the syringe module (2.5 mL syringe and 8-way valve) for wear and tear or contamination.
Renew the syringe if required (see Syringe Maintenance in the Cedex HiRes Analyzer Operator’s Guide).
2
Check the number of runs left. This is listed in the right-hand corner of the Control Center next to a green
or red circle.
3
Check whether there is an empty Cedex Sample Cup in the DefaultCup position on the Multi Sample Tray
or attached to the sample port, for a single sample port.
4
Perform a Prime.
n
2 Prepare the Samples
1
Transfer 300 µl of a well-mixed cell suspension, with a cell concentration in the range of between
5 × 10
4
to 1 × 10
7
cells/mL, to a clean Cedex Sample Cup.
The Cedex HiRes Analyzer tolerates volume variations of +/- 10%. Any greater variation in sample volume
will lead to inaccurate results.
2
Place the prepared Cedex Sample Cup containing the cell suspension in the appropriate cup position on
the Multi Sample Tray or attach to the sample port.
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Check the Analyzer Before Starting Measurements
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