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Cedex HiRes Analyzer Software Version 2.4 - Training Guide
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1.2.4 Exponential Regression Function to Determine the Specific Growth Rate and Doubling
Time of the Cell Culture
Specific growth rate and doubling time for a cell culture are typically calculated based on the change in viable
cell concentration during the log phase of growth. This is the part of the growth curve during which the cell
culture grows most quickly. Since the calculations for specific growth rate and doubling time should only be
carried out using measurement results from the log phase, the measurement results from the lag and plateau
phases need to be removed from the chart before the calculations are performed.
Determine the Specific Growth Rate and Doubling Time of a Culture Based on The Log Phase of
the Growth Curve
1
In the CTC Views dialog box, select the View to be used for the analysis of specific growth rate and
doubling time.
2
Click on the VIEW CHART button.
The Cultivation Time Chart dialog box is displayed showing the change over time in the viable cell
concentration of the cell culture.
3
Study the curve to determine which points on the curve belong to the log phase of growth.
In the figure below, for example, the first three points are most likely part of the lag phase, while the last
three points are most likely part of the plateau phase. These points will need to be deactivated before the
specific growth rate and doubling time can be calculated.
4
In the CTC Views dialog box, select the appropriate View in the top part of the dialog box, then highlight the
relevant series of measurements in the bottom part.
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Monitoring Growth of Cells Over Time
Result Analysis
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