Cedex HiRes User Manual

117
Maintenance and Care
D
D
6.5 Scanning the Chamber to Verify the Effectiveness of Cleaning Routines
The complete flow chamber can be scanned using the SCAN button in the Cleaning dialog box. Using this, the
success of the cleaning can be verified after a cleaning routine.
Proceed as follows:
1
Click on the CLEAN button on the Cedex Control Center to open the Cleaning dialog box.
2
Click on the SCAN button in the lower right-hand corner of the Cleaning dialog box. The flow chamber will
be scanned (i.e., an image of the entire chamber will be created). This process can take 1– 2 minutes during
which the estimated time is displayed in the form of a progression bar.
■
The Current area displays the scanned image.
Since the flow chamber is represented as a whole, it is necessary to enlarge single regions (especially the inlet and
outlet) with the help of the zoom function in order to be able to effectively check the chamber.
Enlarge certain areas of the scan by doing the following:
1
Place the mouse pointer on the top left-hand corner of the area to be enlarged.
2
Hold the left mouse button pressed down and move it to the lower right-hand corner of the area to be enlarged.
3
Release the mouse button and the selected area will fill the whole dialog box.
■
Return to the original size of the scan by following the instructions below:
1
Place the mouse pointer anywhere on the diagram.
2
Hold the left mouse button pressed down and form a box from the bottom right corner to the upper left corner.
3
Release the mouse button. The scan will then return to its original size.
■
The brightness and contrast of the image can also be changed for a better analysis.
If a previous cleaning routine was unsuccessful (i.e., if any (dirt) particles are visible in the Scan image), a new
cleaning of the flow chamber must be done. Perform another scan to check this cleaning. The image created
after this scan can be viewed in the Current area. The image taken previous to this scan can be seen in the
Previous area.
Figure 38: Scanning via the Cleaning dialog box
Daily Maintenance and Cleaning Routines
Scanning the Chamber to Verify the Effectiveness of Cleaning Routines

Cedex HiRes Specifications

General

Viability Range	0 - 100%
Concentration Range	5 x 10^4 to 1 x 10^7 cells/mL
Measurement Principle	Image analysis
Cell Types	Mammalian cells
Measurement Parameters	Total cell count, viable cell count, viability
Measurement Time	Typically < 1 minute
Power Requirements	100-240 V, 50/60 Hz
Interface	USB and Ethernet
Operating Temperature	15-30°C
Operating Humidity	20-80% (non-condensing)