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Table of Contents
Prologue 5
I Document Information .................................................................................................................................................. 5
1 Revision History .................................................................................................................................................................... 5
2 Edition Notice ........................................................................................................................................................................ 5
3 Copyright ................................................................................................................................................................................. 5
4 Trademarks ............................................................................................................................................................................. 5
II Contact Addresses .......................................................................................................................................................... 6
III. Declaration of Conformity ........................................................................................................................................... 6
IV Intended Use ....................................................................................................................................................................... 6
V Preamble ............................................................................................................................................................................... 6
VI Conventions Used in this Guide ............................................................................................................................... 7
VII Warnings and Precautions .......................................................................................................................................... 8
A Startup 10
1 Turning on the Cedex HiRes Analyzer, Control Unit, and Software ..................................................11
2
Preparing the Cedex HiRes Analyzer for Measurements .............................................................................. 12
2.1 Working with the Multi Sampler ..................................................................................................................................12
2.2 Installing a Reagent Kit ...................................................................................................................................................13
2.3 Perform a Prime .................................................................................................................................................................. 14
B Main Application: Counting Cells and Determining Viability 15
1 Check the Analyzer Before Starting Measurements ..................................................................................15
2 Prepare the Samples ....................................................................................................................................................15
3 Setting Up a Single Measurement in the Measurement Dialog Box ................................................16
3.1 Open the Measurement Dialog Box and Define the Sample Parameters .................................................... 16
3.2 Define the Measurement and Analysis Parameters .............................................................................................17
4 Starting the Measurement ........................................................................................................................................ 18
5 End of Measurement ....................................................................................................................................................19
6 Viewing the Results in the Measurement Dialog Box ...............................................................................20
6.1 Viewing the Results in the Measurement Dialog Box .........................................................................................20
6.2 Viewing the Results in the Histograms ......................................................................................................................22
6.3 Viewing the Images ...........................................................................................................................................................22
7 Setting up Multiple Measurements Using the Multi Run Dialog Box ..............................................23
7.1 Open the Multi Run Dialog Box and Prepare Samples for Analysis ..............................................................23
7.2 Define the Sample Parameters and the Measurement and Analysis Parameters
in the Multi Run Dialog Box...........................................................................................................................................24
7.3 Schedule Samples and Start the Measurement ....................................................................................................25
7.4 Interrupting a Multi Run ..................................................................................................................................................26
7.5 Results ...................................................................................................................................................................................26
7.6 The Multi Run Result List................................................................................................................................................27
8 Measurement List ..........................................................................................................................................................28
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