Cedex HiRes User Manual

4
Cedex HiRes Analyzer Software Version 2.4 - Training Guide
Table of Contents
C Additional Applications 29
1 Monitoring Growth of Cells Over Time ...............................................................................................................29
1.1 Performing Measurements for Growth Curve Analysis .......................................................................................29
1.2 Result Analysis ....................................................................................................................................................................29
1.2.1 Create a CTC View .............................................................................................................................................................30
1.2.2 Add One or More Series of Measurements to the CTC View ...........................................................................31
1.2.3 Create a CTC Chart Associated with the View to Analyze Pattern of Growth Over Time .......................34
1.2.4 Exponential Regression Function to Determine the Specific Growth Rate
and Doubling Time of the Cell Culture ......................................................................................................................36
1.2.5 Checking Regression Curve Fitting .............................................................................................................................39
1.2.6 Editing Measurements in a View .................................................................................................................................40
2 Analyzing Unusual Cells .............................................................................................................................................41
2.1 Overview ................................................................................................................................................................................ 41
2.2 Example of Cells Not Recognized by the Cell Type Std. Size ............................................................................42
2.2.1 Create a New Cell Type ...................................................................................................................................................43
2.2.2 Modify the Parameters for the New Cell Type via the Live Operator Dialog Box ......................................45
2.2.3 Reprocess the Measurement Using the New Cell Type ......................................................................................48
D Shutting Down the Cedex HiRes Analyzer 50
1 Perform an LM Shutdown..........................................................................................................................................50
2 Log Out ................................................................................................................................................................................. 50
3 Shut Down the Cedex Control Center ................................................................................................................51
E Index 52

Cedex HiRes Specifications

General

Viability Range	0 - 100%
Concentration Range	5 x 10^4 to 1 x 10^7 cells/mL
Measurement Principle	Image analysis
Cell Types	Mammalian cells
Measurement Parameters	Total cell count, viable cell count, viability
Measurement Time	Typically < 1 minute
Power Requirements	100-240 V, 50/60 Hz
Interface	USB and Ethernet
Operating Temperature	15-30°C
Operating Humidity	20-80% (non-condensing)