Cedex HiRes User Manual

14
Cedex HiRes Analyzer Software Version 2.4 - Training Guide
A
A
9
Place a clean Cedex Sample Cup in the DefaultCup position on the Multi Sample Tray or attach it to the
single sample port, for a system with a single sample port.
Perform a Prime (see Perform a HW Startup and Prime, below).
For a system with a single sample port, ensure that a Cedex Sample Cup is always attached to the single
sample port. The Cedex Sample Cup (4 mL) is correctly attached when it is in an upright position beneath
the single sample port, and the capillary tube almost reaches the bottom of the Cedex Sample Cup:
n
2.3 Perform a Prime
Prime fills the capillary tubes and 8-way valve with the appropriate reagents. HW Startup initializes hardware
interfaces such as the scanner, Multi Sampler, and pump. By default, the software automatically performs an
HW Startup when launched (see Automatically Start the Hardware Management in the Cedex HiRes Analyzer
Operator’s Guide).
Both the Prime and the HW Startup functions are located in the Hardware Management dialog box.
1
Click the HWM button in the Cedex Control Center or select Hardware Management under the Functions
menu. The Hardware Management dialog box opens.
2
Ensure that a Cedex HiRes Reagent Kit is installed (see Installing a Reagent Kit) and a clean, empty Cedex
Sample Cup is correctly positioned on the Multi Sample Tray in the DefaultCup position or attached to the
single sample port.
3
Click the PRIME button in the Hardware Management dialog box. The duration of a Prime procedure is
displayed in the Estimated total time area. The message “HW started and LM primed” appears in the current
status field when the Prime is completed.
If the Hardware Management has not been started, the message “HW not started and LM not primed”
appears in the current status field. Click on the HW STARTUP button if this message appears.
4
Click on to close the dialog box.
n
There should not be a break of more than 2 hours between a Prime and a measurement. If more than 2 hours
have passed since the last measurement, perform a new Prime.
Preparing the Cedex HiRes Analyzer for Measurements
Perform a HW Startup and Prime

Cedex HiRes Specifications

General

Viability Range	0 - 100%
Concentration Range	5 x 10^4 to 1 x 10^7 cells/mL
Measurement Principle	Image analysis
Cell Types	Mammalian cells
Measurement Parameters	Total cell count, viable cell count, viability
Measurement Time	Typically < 1 minute
Power Requirements	100-240 V, 50/60 Hz
Interface	USB and Ethernet
Operating Temperature	15-30°C
Operating Humidity	20-80% (non-condensing)