17
Main Application: Counting Cells and Determining Viability
B
B
3.2 Define the Measurement and Analysis Parameters
1
Manually enter the cup position for the sample in the Cup Position field if there is a Multi Sampler.
The positions are marked on the Multi Sample Tray.
2
In the Processing Parameters group of the Measurement dialog box, select the desired precision level in the
Precision list.
The precision level determines the volume to be analyzed. A higher analyzed volume requires a somewhat
longer measurement time but more cells will be counted:
c
Minimum: approximately 3 images are used for the analysis.
c
Normal: approximately 6 images are used for the analysis.
c
Superior: approximately 8 images are used for the analysis.
c
Maximum: approximately 11 images are used for the analysis.
3
Select the Cell Type that optimally recognizes your cells from the Cell Type list. The following Cell Types
are available by default:
c
Min. Size for small cells with a diameter of about 6 µm, such as lymphocytes; default sedimentation
time is 90 seconds.
c
Std. Size for normal-sized cells with a diameter of about 12 µm, such as HB58; default sedimentation
time is 60 seconds.
c
Max. Size for large cells of more than 16 µm, such as K-562; default sedimentation time is 60 seconds.
If a new Cell Type has been created, it can also be selected from the same Cell Type drop-down list.
Do not select the Cell Type option Illumination Test. This Cell Type is designed for service purposes
only and is not intended for analysis of cells. The parameters for this Cell Type should not be changed.
4
The Cedex Control Center automatically adds the:
c
System Name
c
Process Time
5
Prepare the sample and place the Cedex Sample Cup containing the sample in the appropriate position on
the Multi Sample Tray or attach it to the single sample port.
The Sample Port area indicates which type of sample port is used for the measurement, based on the
instrument configuration (see the Cedex HiRes Analyzer Operator’s Guide for more information about the
instrument configuration):
c Multi Sampler: use the Multi Sample Tray.
c Single Port: use the single sample port.
c
Remote Port: use the Remote Sample Port located at the top of the instrument (see Chapter A).
c
Custom Port: Consult with your Cedex administrator for more information about which sample
port to
use before running any measurements.
n
Setting Up a single Measurement in the Measurement Dialog Box
Define the Measurement and Analysis Parameters
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