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Software
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10.4.2. Turning off Automatic Incrementation of Sample Cup Position
In the case of single measurements run via the Measurement dialog box on a HiRes system with a Multi Sampler
attached, the Software will automatically incrementally increase the sample cup position number with each new
measurement. To turn off this feature:
1
Open the Systems Options dialog box and click on the Measurement tab.
2
Look for the parameter SuggestNextCupPosition.
3
Select 0 from the drop-down menu to turn off the feature.
4
Click on Save to confirm the change.
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When 0 is selected, the suggested cup position will always be the same.
10.4.3. Turning off the Flow Chamber Prescan
Only cells that have settled down on the bottom of the chamber are well inside the focal plane of the scanner
and, therefore, can be analyzed with an optimal image quality. A default sedimentation time of 60 or 90 seconds,
depending on Cell Type, was selected to ensure that all of the cells from different cell types have settled down to
the bottom of the flow chamber. Cells with larger diameters will generally settle to the bottom within 60 seconds.
However, many cells with smaller diameters may require more time. Reducing the amount of time set for the
sedimentation time will reduce the overall measurement time. Reducing the sedimentation time, however, is
not standard procedure. A specific verification process is required to confirm the correct sedimentation time for
a particular cell line. Consult a local Roche Service representative for detailed information on the verification
process.
During the sedimentation phase, a Prescan of the flow chamber is done and an image of the whole flow chamber
delivered. If the sedimentation time is reduced to below 60 seconds, the flow chamber Prescan may need to be
disabled since there may no longer be sufficient time for a Prescan to be taken.
To disable the Flow Chamber Prescan, do the following:
1
Open the System Options and click on the Measurement tab.
2
Look for the parameter MeasurementPrescanRequired. Enter 0 in the Value column to disable the Prescan.
3
Click on Save to store the change.
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If the Prescan should be enabled again, re-enter 1 in the Value column of the parameter
MeasurementPrescan-
Required.
The Cedex HiRes Software requires one Prescan image to optimize the image brightness. It is necessary
to ensure that at least one Trypan Blue-stained sample has been run on the system before turning off the
Prescan feature.
The 60 second sedimentation time set by default for the Std. Size and Max. Size should generally allow
enough time for a Prescan to be performed. For particularly slow computer systems, however, the Prescan
may require slightly more time than the default sedimentation time. If this occurs, a message “Warning:
sedimentation time was exceeded” will appear in the main Cedex Control Center. However, there will be no
effect on the measurement other than slightly longer sedimentation time. The sedimentation time for the Std.
Size and Max. Size can be increased, if desired, in order to have a sedimentation time that is longer than the
Prescan time (see “Editing an Existing Cell Type”).
Administrator Functions
The System Options
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