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Cedex HiRes - User Manual

Cedex HiRes
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Cedex HiRes Analyzer Operators Manual
Software Version 2.4
October 2017

Table of Contents

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Summary

How to Use the Cedex HiRes Analyzer Guides

Addenda

Provide updates to the Cedex HiRes Analyzer Guides, including new supplementary information and corrections to previous editions.

Cedex HiRes Analyzer Guides Overview

Overview of available guides: User Training, Quick, and Operator's Guides.

Prologue

Document Information

Revision history, edition notice, copyright, trademarks.

Contact, Compliance, and Intended Use

Contact details, regulatory compliance, warranty, and scope of Analyzer's use.

Software Disclaimers and License

Legal disclaimers and terms for using the Cedex HiRes Software.

Conventions and Safety Precautions

Guide conventions, text formatting, symbols, and essential safety instructions.

Startup

Powering On and Initializing the System

Turning on Analyzer, Control Unit, and launching Cedex Software.

Analyzer Preparation for Measurements

Setting up the Analyzer: Multi Sampler, Reagent Kit installation, and Prime procedure.

Main Application: Counting Cells and Determining Viability

Pre-Measurement Checks and Sample Preparation

Verifying Analyzer status and preparing cell suspension samples.

Single Measurement Setup and Execution

Configuring measurement parameters and initiating a single sample analysis.

Results Viewing, Analysis, and Data Management

Interpreting results, viewing images, analyzing data via histograms, and managing data.

Multi Run Measurement Setup and Execution

Configuring and running batch measurements using the Multi Run dialog.

Additional Applications: Growth Curve Analysis

Monitoring Cell Growth Over Time

Performing measurements at intervals to track cell culture growth patterns.

Cultivation Time Chart (CTC) Analysis

Creating charts, analyzing growth data, calculating growth rate and doubling time.

Analyzing Unusual Cells

Cell Type Creation and Adjustment

Defining custom Cell Types and optimizing image analysis parameters.

Reprocessing Measurements with New Cell Types

Applying custom Cell Types to existing data sets for re-analysis.

Shutting Down the Cedex HiRes Analyzer

System Shutdown Procedures

Sequence for shutting down Analyzer hardware and software components.

Performing a Measurement

System Startup and Preparation

Turning on the system and preparing it for measurements.

Single Measurement Setup and Execution

Configuring and initiating a single sample analysis.

Sample Preparation and Shutdown

Preparing samples and performing system shutdown procedures.

Working with the Multi Sampler

Multi Sampler Components and Tray Usage

Details on the DefaultCup and Multi Sample Tray usage.

Multi Run Measurement Setup and Execution

Configuring and running batch measurements using the Multi Run dialog.

Data Entry, Results, and Batch Management

Editing data, viewing results, and managing batch processes.

Overview

Analyzer Description and Measurement Procedure

Description of Analyzer components and the Trypan Blue Exclusion Method.

Data Analysis and Technical Specifications

Software data analysis capabilities and detailed Analyzer technical specifications.

System Description

Analyzer Installation and Location

Steps for Analyzer installation and selecting an appropriate operating location.

Hardware Connections and Power Management

Connecting system components and managing power supply.

System Startup and Software Access Control

Starting the system, logging in, and managing OS-level access controls.

Cedex HiRes Software Access Control

Managing user access levels and passwords within the Cedex HiRes Software.

Software Functions

Cedex Control Center Interface

Navigating the Control Center: menus, shortcuts, and event tracking.

Measurement Operations

Configuring, starting, interrupting, and concluding single measurements.

Multi Run Operations and Data Exchange

Managing batch measurements, data exchange, and related processes.

Results Viewing and Graphical Analysis

Interpreting results, viewing images, and analyzing data via histograms.

Cultivation Time Chart (CTC) Analysis

Analyzing cell growth patterns using CTCs, including regression and offset adjustments.

System Functions and Data Management

Printing reports, reviewing audit trails, and managing system data.

User Preferences and System Configuration

Customizing user settings, administrator functions, and system options.

Image Analysis Adjustment

Adjusting image analysis parameters and creating custom Cell Types.

Maintenance and Care

Cleaning Routines and Verification

Scheduling, performing, and verifying various cleaning routines.

Liquid Management System Operations

Performing Prime and LM Shutdown routines via Hardware Management.

Reagent Kit Management

Installing kits and refilling reagent containers for optimal performance.

Calibration and System Suitability Testing (SST)

Performing adjustments, SSTs, tracking results, and managing system lock status.

Syringe Maintenance

Procedures for removing, installing, and maintaining the syringe module.

Fuse Exchange and Component Maintenance

Replacing fuses and preventative maintenance of key hardware components.

Operational Data

Monitoring system operational data.

Troubleshooting

Diagnosis and Audit Trail

Generating diagnostic files and reviewing system audit trails for issue resolution.

System Reboot Procedures

Step-by-step instructions for rebooting the Analyzer and Control Unit.

Appendix

System Options and User Preferences

Configuring system options and customizing user preferences.

General, Measurement, and List Settings

Configuring general software options, measurement defaults, and list filters.

Administrator Settings and Security

Configuring hardware, reports, XML, password policies, and focus adjustment.

Summary

How to Use the Cedex HiRes Analyzer Guides

Addenda

Provide updates to the Cedex HiRes Analyzer Guides, including new supplementary information and corrections to previous editions.

Cedex HiRes Analyzer Guides Overview

Overview of available guides: User Training, Quick, and Operator's Guides.

Prologue

Document Information

Revision history, edition notice, copyright, trademarks.

Contact, Compliance, and Intended Use

Contact details, regulatory compliance, warranty, and scope of Analyzer's use.

Software Disclaimers and License

Legal disclaimers and terms for using the Cedex HiRes Software.

Conventions and Safety Precautions

Guide conventions, text formatting, symbols, and essential safety instructions.

Startup

Powering On and Initializing the System

Turning on Analyzer, Control Unit, and launching Cedex Software.

Analyzer Preparation for Measurements

Setting up the Analyzer: Multi Sampler, Reagent Kit installation, and Prime procedure.

Main Application: Counting Cells and Determining Viability

Pre-Measurement Checks and Sample Preparation

Verifying Analyzer status and preparing cell suspension samples.

Single Measurement Setup and Execution

Configuring measurement parameters and initiating a single sample analysis.

Results Viewing, Analysis, and Data Management

Interpreting results, viewing images, analyzing data via histograms, and managing data.

Multi Run Measurement Setup and Execution

Configuring and running batch measurements using the Multi Run dialog.

Additional Applications: Growth Curve Analysis

Monitoring Cell Growth Over Time

Performing measurements at intervals to track cell culture growth patterns.

Cultivation Time Chart (CTC) Analysis

Creating charts, analyzing growth data, calculating growth rate and doubling time.

Analyzing Unusual Cells

Cell Type Creation and Adjustment

Defining custom Cell Types and optimizing image analysis parameters.

Reprocessing Measurements with New Cell Types

Applying custom Cell Types to existing data sets for re-analysis.

Shutting Down the Cedex HiRes Analyzer

System Shutdown Procedures

Sequence for shutting down Analyzer hardware and software components.

Performing a Measurement

System Startup and Preparation

Turning on the system and preparing it for measurements.

Single Measurement Setup and Execution

Configuring and initiating a single sample analysis.

Sample Preparation and Shutdown

Preparing samples and performing system shutdown procedures.

Working with the Multi Sampler

Multi Sampler Components and Tray Usage

Details on the DefaultCup and Multi Sample Tray usage.

Multi Run Measurement Setup and Execution

Configuring and running batch measurements using the Multi Run dialog.

Data Entry, Results, and Batch Management

Editing data, viewing results, and managing batch processes.

Overview

Analyzer Description and Measurement Procedure

Description of Analyzer components and the Trypan Blue Exclusion Method.

Data Analysis and Technical Specifications

Software data analysis capabilities and detailed Analyzer technical specifications.

System Description

Analyzer Installation and Location

Steps for Analyzer installation and selecting an appropriate operating location.

Hardware Connections and Power Management

Connecting system components and managing power supply.

System Startup and Software Access Control

Starting the system, logging in, and managing OS-level access controls.

Cedex HiRes Software Access Control

Managing user access levels and passwords within the Cedex HiRes Software.

Software Functions

Cedex Control Center Interface

Navigating the Control Center: menus, shortcuts, and event tracking.

Measurement Operations

Configuring, starting, interrupting, and concluding single measurements.

Multi Run Operations and Data Exchange

Managing batch measurements, data exchange, and related processes.

Results Viewing and Graphical Analysis

Interpreting results, viewing images, and analyzing data via histograms.

Cultivation Time Chart (CTC) Analysis

Analyzing cell growth patterns using CTCs, including regression and offset adjustments.

System Functions and Data Management

Printing reports, reviewing audit trails, and managing system data.

User Preferences and System Configuration

Customizing user settings, administrator functions, and system options.

Image Analysis Adjustment

Adjusting image analysis parameters and creating custom Cell Types.

Maintenance and Care

Cleaning Routines and Verification

Scheduling, performing, and verifying various cleaning routines.

Liquid Management System Operations

Performing Prime and LM Shutdown routines via Hardware Management.

Reagent Kit Management

Installing kits and refilling reagent containers for optimal performance.

Calibration and System Suitability Testing (SST)

Performing adjustments, SSTs, tracking results, and managing system lock status.

Syringe Maintenance

Procedures for removing, installing, and maintaining the syringe module.

Fuse Exchange and Component Maintenance

Replacing fuses and preventative maintenance of key hardware components.

Operational Data

Monitoring system operational data.

Troubleshooting

Diagnosis and Audit Trail

Generating diagnostic files and reviewing system audit trails for issue resolution.

System Reboot Procedures

Step-by-step instructions for rebooting the Analyzer and Control Unit.

Appendix

System Options and User Preferences

Configuring system options and customizing user preferences.

General, Measurement, and List Settings

Configuring general software options, measurement defaults, and list filters.

Administrator Settings and Security

Configuring hardware, reports, XML, password policies, and focus adjustment.

Cedex HiRes Specifications

General IconGeneral
Viability Range0 - 100%
Concentration Range5 x 10^4 to 1 x 10^7 cells/mL
Measurement PrincipleImage analysis
Cell TypesMammalian cells
Measurement ParametersTotal cell count, viable cell count, viability
Measurement TimeTypically < 1 minute
Power Requirements100-240 V, 50/60 Hz
InterfaceUSB and Ethernet
Operating Temperature15-30°C
Operating Humidity20-80% (non-condensing)