Cedex HiRes - Reprocessing Measurements with New Cell Types

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Cedex HiRes Analyzer Software Version 2.4 - Training Guide

Make the desired modiﬁcations using the sliders.

In the example shown in the previous screenshot, some live and dead cells were not recognized. This is most

likely due to the fact that some of the cells fell below the cutoff for minimum size and also had an unusually

weak contrast against the background.

For this particular cell culture, the following changes were made to the Cell Type New Cell Type (for more

detailed information about available parameters, see the Cedex HiRes Analyzer Operator’s Guide):

c CMinSize set to: 4

c LCAppearance set to: 10

c DCAppearance set to: 10

Image analysis with Std. Size before parameter

adjustments.

Image analysis with New Cell Type after parameter

adjustments.

The labeling of the cells can be switched on and off using the checkboxes in the upper-right-hand corner

in order to allow for better evaluation of the results from changes in parameter settings.

As soon as each parameter is changed, the data is automatically reprocessed. If multiple parameter changes

are made at the same time, remove the checkmark in the Automatic process box by clicking on it. When

the checkmark is removed, the button Process now becomes active. Make all changes, and then click on

the Process now button for an analysis based on the new parameter settings. The image display shows the

reprocessed image, as well as changes in the number of live and dead cells identiﬁed by the new Cell Type:

c The values New Viable Cells (the number of living cells in the current image) and New Dead cells (the

number of dead cells in the current image) are displayed in the New column of the Process Data area.

c If an Operator parameter is modiﬁed, the newest values will appear in the New column after the

automatic reprocessing of the selected image is ﬁnished.

c The values of the previous processing are displayed in the Previous column.

c In addition, Viability [%] in the New column shows the viability of the last processing of the image, while

Viability [%] in the Previous column shows the viability of the second-to-last processing of the image.

Thus, it is possible to directly compare the evaluation with each previous evaluation. By activating the Fixed

checkbox in the Process Data area, the values currently displayed in the Previous column will not be changed

after further adjustments of operator parameters. This allows, for example, the comparison of evaluations

with the original evaluation when activating the checkbox after the ﬁrst parameter adjustment. By clicking

the Show original process parameters button you can see the process parameters originally used.

ccc

Analyzing Unusual Cells

Example of Cells Not Recognized by the Cell Type Std. Size