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Cedex HiRes Analyzer Operator’s Guide
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If a reagent is completely used up during measurements and then refilled, a Prime must be performed again
before running the next sample (see “The Hardware Management Dialog Box”). If a Prime is not performed,
the Prescan of the first measurement after refilling the reagent will fail due to the presence of bubbles, and
the measurement will be considered not valid by the software (there will be no check in the ‘Valid’ checkbox
found in the Measurement dialog box (see “Viewing the Prescan Image”). Subsequent measurements will not
have bubbles present in the Prescan.
For reliable functioning of the reagent control (correct number of possible measurements, or reporting an
impending overflow of the waste container), ensure that all reagent containers are correctly filled to the line
with the appropriate liquid or, for the waste container, completely emptied.
Confirm the changing of the reagents in the Software as soon as the Reagent Tray has been put back into the
chamber. Failure to do all of the above will result in a mismatch between the calculated reagents available as
shown in the Reagent Control and the actual reagent amounts in the containers or space available in the waste
container.
The reagent control function that monitors reagent and waste levels can be turned off. See “Disabling the
Reagent Control”.
Cleaning the reagent containers when manually refilling
All reagent containers must be thoroughly cleaned at regular intervals and rinsed with distilled or deionized
water. Impurities caused by dust or fluff must absolutely be avoided. The Trypan Blue is especially critical as it
can become contaminated with microorganisms. If contaminated, exact measurements are no longer possible.
If the Trypan Blue solution is contaminated, intense areas may be visible in the image that will make image
analysis difficult or even impossible. Replacing the reagents after 5 days is recommended.
Checking the reagent containers when manually refilling
Empty reagent containers lead to wrong measurement results and can cause a dry run, thereby damaging the
syringe and the 8-way valve. Regular checks of the liquid level are done via the Reagent Kit Status dialog box.
For safety reasons, the liquid level of the individual reagent containers, as well as the capillary positions, should
also be manually checked before each measurement and, if necessary, replaced or refilled.
Installing or Changing the Reagent Kit
Refilling Containers Individually
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