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Maintenance and Care
D
D
Standard Clean (with cleaning solution, detergent, and distilled water for approximately 90 seconds)
The entire Liquid Management system is rinsed with distilled water, cleaning solution, detergent, and finally
with distilled water again. This routine removes dirt particles and is used after measuring a set of samples or
before a longer pause.
Intensive Clean (with cleaning solution, detergent, and distilled water for approximately 20 minutes)
The flow chamber is thoroughly rinsed with distilled water, cleaning solution, distilled water, detergent, and
finally distilled water. The detergent remains in the flow chamber for a longer period to work on resistant
particles.
The Intensive Clean routine is used on weekly basis to remove heavy and resistant contamination of the flow
chamber, capillaries tubes, syringe, and 8-way valve. Even the smallest dirt particle attached to the glass wall
of the flow chamber can negatively influence a measurement. See also “Scanning the Chamber to Verify the
Effectiveness of Cleaning Routines”. If measurement values within a sample measurement show large variations,
this could indicate that the flow chamber is soiled.
Intensive Clean with HCl
A special Intensive Clean is required twice per month for a thorough cleaning. For this cleaning, a solution of
0.1 to 1 N HCl is used instead of detergent.
Follow the instructions below:
1
Remove the Detergent container and replace it with an appropriate container with a 0.1 to 1 N HCl solution,
taking care that the capillary tube reaches far enough into the solution.
2
Start Prime.
3
Start Intensive Clean after Prime has ended.
4
Replace the HCl container with the Detergent container at the end of the Intensive Clean process.
5
Start Prime again.
6
Start Standard Clean after Prime has ended.
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The Chamber Clean 1 and Chamber Clean 2 cleaning routines are only required for the flow chamber.
Chamber Clean 1 (with water for approximately 10 seconds)
The flow chamber is rinsed at high pressure with distilled or deionized water being drawn in through the
syringe. Use this routine to remove resistant protein aggregates, fluff particles or other obstructions from the
flow chamber.
Chamber Clean 2 (with water, detergent, and air for approximately 30 seconds)
This routine is more intense than the Chamber Clean 1 procedure. Once the flow chamber is rinsed with water
and detergent, the remaining liquid in the capillary tubes between the syringe and valve head is drawn through
the flow chamber and then pressed back again with compressed air.
Use this routine twice daily to remove resistant protein aggregates, fluff particles or other obstructions from the
flow chamber.
Daily Maintenance and Cleaning Routines
Cleaning Options
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