AS Autosampler Operator’s Manual
160 Doc. 065051-03 1/08
• Step 2: Clicking Setup empties the needle seal line and the diverter line.
• Step 3: Enter an approximate volume using the following formula:
0.8
μL/in (2 μL/in) for the diverter line plus the volume of the needle seal
line (which is written on a label on the tubing and inside the AS front
door). Then, click Estimate. The AS dispenses the volume entered, which
nearly fills the diverter and needle seal lines (minus a few microliters).
NOTE The value used for approximating the diverter line
volume (0.8 μL/cm, 2 μL/in) assumes a tubing ID of
0.330 mm (0.130 in). For volume per length values for
other tubing IDs, see Section 3.8
.
• Step 4: After clicking +1 uL, wait until the sound of the syringe motor
stops before clicking +1 uL again.
Continue clicking +1 uL until a tiny drop of liquid appears at the end of
the diverter line. If you go too far and more than a single small drop of
liquid appears, click -5uL. The AS will empty the line and then fill the
line with 5
μL less than was cleared. You can then begin clicking +1 uL
again.
• Step 5: After clicking Test, verify that a drop of liquid appears at the end
of the diverter line. If no drop appears or if the drop is a different size than
previously observed, click -5uL and repeat the procedure.
• Step 6: After clicking Done, the new calibration value is displayed on the
panel. Copy this value to the label inside the AS front door.
• Calibrate System 2: Select Diverter Valve Position_2 and repeat the
calibration procedure for system 2.
• Finishing: Reconnect the diverter lines to the injection valves. Tighten
the fittings fingertight and then tighten an additional one-quarter turn
using the 5/16-in wrench.
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