Dionex As - Page 302

AS Autosampler Operator’s Manual
290 Doc. 065051-03 1/08
Reagent Flush after Concentrator Loading or Reagent Priming
REAGENT FLUSH is used to flush reagent onto the concentrator column
after sample is loaded. See Section 3.6.10
for details about concentrator
injections.
The following steps occur during reagent flush:
• (Optional) The inject valve is switched to a specified position (load or
inject). For most applications, the valve position is not changed.
• If the reagent source is a vial, the needle arm moves to the vial and
picks up the volume of reagent (see the notes below for volume
ranges and defaults).
• The needle arm moves to the inject port.
• The syringe delivers the specified volume of reagent from the
reservoir to the inject port. From there it moves onto the concentrator.
The sample syringe and the flush reservoir are used by default for
reagent flushing. If the sample prep option is installed, the prep
syringe is used and a reagent reservoir can be specified.
Notes on reagent prime and flush volume ranges:
• If the source is a reservoir, the allowed range is 100 to 32,600 μL. The
default is 2,000
μL if the syringe volume is less than 5,000 μL, and
10,000
μL if the syringe volume is 5,000 or 10,000 μL.
1000
uL VlvFLH N
RGNT_FLH
Reagent Source
I
n
j
ect
V
a
l
ve
P
os
i
t
i
on
Volume to Flush
Volume
Vial or flush
reservoir. Also,
RES A, B, C, or D,
if sample prep is
installed
L (load)
I (inject)
N (no change)
100.0 to
50,000 L
(see notes)
μ
Source
Inject Valve