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Dionex As
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Doc. 065051-03 1/08 289
Reagent Prime after Concentrator Loading
NOTE
REAGENT PRIME and REAGENT FLUSH are
included in the sample prep steps. However, they are
not performed until after the Load (concentrate)
step in the timed events (see Section D.5.5
).
REAGENT PRIME is used to prime the sampling needle assembly with
reagent after sample has been loaded onto a concentrator column. See
Section 3.6.10
for details about concentrator injections. See Section 2.3.3
for details about the sampling needle assembly.
The following steps occur during reagent prime:
(Optional) The inject valve is switched to a specified position (load or
inject). For most applications, the valve position is not changed.
The needle arm moves to the waste port.
The prep syringe delivers the specified volume of reagent from the
reagent reservoir. This primes the sampling needle assembly with
reagent.
NOTE Although it is possible to select a vial or the flush
reservoir as the reagent source,
REAGENT PRIME is
designed to be used only when a reagent reservoir is
the selected reagent source and the prep syringe is
installed.
10000
uL Vlv#1 N
RGNT_PRM
Reagent
S
ource
I
n
j
ect
V
a
l
ve
P
os
i
t
i
on
V
o
l
ume
to
P
r
i
me
Volume
L (load)
I (inject)
N (no change)
100.0 to
50,000 L
(see notes)
μ
Source
Inject Valve
RES A, B, C, or D
(Requires sample
prep option)

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