2 • Description
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Each injection in a schedule is assigned a method (a series of operating
instructions that tells the AS how to perform a single injection). A method
consists of the following three phases:
When the method runs, the
AS performs the sample prep steps first,
followed by the method setup, and then the timed events steps.
Section D.5
describes how to create methods.
Notes
• See Appendix D for details about front panel operation.
• Some autosampler operations are not available from the AS front
panel and can only be accessed from Chromeleon or Chromeleon
Xpress. These include setting different aspirate and dispense syringe
speeds and sampling from well plates.
Method Phase Used To Examples
Sample prep Prepare the sample for injection Pipetting, mixing, diluting
Method setup Set parameters that remain
constant throughout the method
Sample needle height, sample
temperature
Timed events Perform functions at a specific
time in the method
Loading the sample loop,
injecting
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