3 • Operation and Maintenance
Doc. 065051-03 1/08 85
Example Program for Concentrate Mode (Matrix Elimination)
The following example Chromeleon or Chromeleon Xpress program
shows the AS commands when the autosampler is in concentrate mode.
Commands similar to these could be used for a matrix elimination
application.
Flush Volume = 100 ;AS options commands
Wait FlushState
NeedleHeight = 2
CutSegmentVolume = 0
SyringeSpeed = 3
CycleTime = 0
Pipet Volume = 20.0, SourceVial =
CurrentVial, DestinationVial =
CurrentVial
;AS sample prep
commands
Mix SourceVial = CurrentVial,
NumberOfTimes = 5, Volume = 250.0
FlushSP Volume = 250.0
DelaySP Time = 0.0
Dispense Volume = 1000.0,
SourceReservoir = Reservoir_A,
DestinationVial = CurrentVial
WaitForTemperature = False
Concentrate ValvePosition =
LoadPosition, AspirateSpeed = 3,
DispenseSpeed = 1
;Switch the inject valve
to Load and load sample
onto the concentrator
ReagentPrime Volume = 10000.0,
SourceVial = Reservoir_A, ValvePosition
= NoChange
;Prime sampling needle
assembly with reagent
ReagentFlush Volume = 5000.0, SourceVial
= Reservoir_A, ValvePosition = NoChange
;Flush concentrator with
reagent; this is the
matrix elimination step
Wait SampleReady
0.000 Wait CycleTimeState
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