5•Service
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Notes for calibrating the inject port volume when in normal mode:
• Preparing: After disconnecting the needle seal line, route the line outside
the autosampler or chromatography compartment.
Make sure the sample syringe is free of bubbles (see Section B.3.14
).
• Step 1: Clicking Setup first empties the needle seal line. The AS then
dispenses 25
μL into the inject port. This nearly fills the needle seal line
(minus a few microliters).
Figure 5-27. Chromeleon Inject Port Volume Calibration Panel: Normal Mode
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