AS Autosampler Operator’s Manual
158 Doc. 065051-03 1/08
• Step 2: After clicking +1 uL, wait until the sound of the syringe motor
stops before clicking +1 uL again.
Continue clicking +1 uL until a tiny drop of liquid appears at the end of
the needle seal line. If you go too far and more than a single small drop of
liquid appears, click -5uL. The AS will empty the line and then fill the
line with 5
μL less than was cleared. You can then begin clicking +1 uL
again.
• Step 3: Press Reset and then Test. After clicking Test, verify that a drop
of liquid appears at the end of the needle seal line. If no drop appears, or if
the drop is a different size than previously observed, click -5uL and
repeat the procedure.
• Step 4: After clicking Done, the new calibration value is displayed on the
panel. Write this value on the label inside the AS front door.
• Finishing: Reconnect the needle seal line to the injection valve. Tighten
the fitting fingertight and then tighten an additional one-quarter turn using
the 5/16-in wrench.
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