Dionex As - Page 325

D • Front Panel Control
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7. Select the following options:
ASPIRATE: 60 μL for normal, simultaneous, and concentrate modes; 120 μL
for sequential and sequential concentrate modes (to empty the needle seal line
and diverter tubing)
from NEEDLE
SYRINGE SPEED: 3
ACTION
: ASPIRATE
8. Press Enter.
9. Select
VIAL#: FLU and press Enter.
The needle arm moves to the flush port.
10. Select
ACTION: EMPTY and press Enter.
11. Select the following options:
from FLUSH
SYRINGE SPEED
: 5
ACTION
: FILL
12. Press Enter.
13. Select the following options:
DISPENSE: 50 μL
SYRINGE SPEED: 5
ACTION
: DISPENSE
14. Press Enter.
15. Select
VIAL#: INJ and press Enter.
The needle arm moves to the inject port.
16. Select the following options:
DISPENSE: 25 μL for normal mode; 75 μL for sequential mode
SYRINGE SPEED: 1
ACTION
: DISPENSE
17. Press Enter.
The syringe dispenses 25
μL or 75 μL.