AS Autosampler Operator’s Manual
314 Doc. 065051-03 1/08
18. Select the following options:
DISPENSE: 1 μL
SYRINGE SPEED: 1
ACTION
: DISPENSE
19. Press Enter.
The syringe dispenses 1
μL.
20. Continue pressing
Enter, counting each key press, until a tiny drop of liquid
appears at the end of the needle seal line. After each key press, wait until
the sound of the syringe motor stops before pressing
Enter again.
21. Record the volume entered (including the volume in Step 17
and Step 19).
Also, notice the size of the liquid drop. This will be compared to the size of
the liquid drop in Step 34
.
22. Select the following options:
ASPIRATE: 60 μL for normal mode; 120 μL for sequential mode (to empty the
needle seal line and diverter tubing)
from NEEDLE
SYRINGE SPEED
: 3
ACTION
: ASPIRATE
23. Press Enter.
24. Select
VIAL#: FLU and press Enter.
The needle arm moves to the flush port.
25. Select the following options:
SYRINGE SPEED: 5
ACTION: EMPTY
26. Press Enter.
27. Select the following options:
from FLUSH
SYRINGE SPEED: 5
ACTION
: FILL
28. Press Enter.
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