3 • Operation and Maintenance
Doc. 065051-03 1/08 79
Setting Up a Simultaneous Injection
The AS must be equipped with the simultaneous injection option in order
to perform simultaneous injections. The following setup steps are
required:
• Follow the instructions in Section B.3.5 to connect the AS to two
injection valves (either in the autosampler compartment or two IC
systems). In this configuration, the inject port tubing is connected to a
Y-connector and the legs of the Y are connected to the inject valves in
both systems (see Figure 3-23
). The tubing from the Y to each valve
must be the same length.
• Install a sample syringe of 5,000 or 10,000 μL.
• Enter the sample syringe size of 5,000 or 10,000 μL on the
PLUMBING CONFIGURATION screen (see Section C.6.3).
• On the SYSTEM PARAMETERS screen (see Section C.6.5), select
SIMULTANEOUS in the SAMPLE MODE field.
• In the Chromeleon or Chromeleon Xpress sequence (see Figure 3-19)
or in the front panel schedule (see Section D.3
), enter an injection
volume that is large enough to allow flushing the inject port, flushing
the tubing from the inject port to each valve (25
μL per inch of
tubing), plus enough to flush and fill both injection loops (this is
about 5 times the combined loop sizes).
Example Simultaneous Injection Parameters:
45
μL = inject port volume
500
μL = 20 inches of connector tubing (combined from both
systems) x 25
μL
625
μL = 5 x (100 μL loop #1 + 25 μL loop #2)
total = 1170
μL
The specified injection volume is loaded into the sample syringe and
then delivered equally to the two sample loops.
To Next Page
Loading...
